The Little Engine Shop is the web site of Jeff Gelles’ laboratory in the Department of Biochemistry at Brandeis University.  We study “little engines”, the nanometer-sized machines made of protein, RNA, and DNA molecules that carry out essential processes in living cells.  A central focus of the lab is the use of single-molecule light microscopy methods to study the functional mechanisms of these macromolecular complexes in cytoskeletal function, transcription and transcription regulation, and pre-messenger RNA splicing. The research we do encompasses fields from cell and molecular biology through biochemistry and biophysics to physical chemistry and condensed matter physics.