What happens when good genes get lost?

Scientifically speaking, there is no bad DNA, though we like to blame it for unruly hair, klutziness or poor gardening skills. There is, however, junk DNA.

Heterochromatin are tight bundles of DNA that often contain the genes our cells want to keep quiet, either because they are not needed or they are repetitive. Heterochromatic genes are silenced by a muzzle-like protein while the gene cells we want expressed, called euchromatin, are activated.

Sometimes, an important gene — a gene we want activated — gets mistakenly embedded in junk DNA. Scientists have long wondered how those genes get activated. Now, Brandeis University researchers may have solved that mystery.

Michael Marr, associate professor of biology, published a paper in the journal Molecular and Cellular Biology this summer with John Lis of Cornell University, Jessica Treisman of the NYU School of Medicine and former Brandeis research associate Sharon Marr, now at the Massachusetts General Hospital.

Hp1 Med26
Polytene chromosome stains

Transcription of DNA into RNA is an insanely complicated process that is still not entirely understood. In the 1990s, Roger Kornberg, a professor of structural biology at Stanford University, discovered a huge, multiprotein complex he called Mediator, which helps activate transcription. Kornberg won the Nobel Prize for his discovery.

Mediator, which is present in every eukaryotic organism from yeast to humans, is composed of dozens of subunits. However, some of the subunits are found only in animal cells. One of these subunits, Med26, has long been thought to be a marker for active, euchromatin genes.

Yet Marr and his colleagues discovered Med26 in heterochromatin — the first time the subunit has been observed among inactive genes. Either Med26 is moonlighting in an entirely new role, says Marr, or it is there tracking down lost genes and activating them.

“It looks as though Med26 is tethered to the silencer and maybe activates essential genes that got lost in the junk pile,” Marr says.

Marr and his team studied Med26 in Drosophila, the first time the subunit has been studied in an intact animal model. The team discovered that while cells and organs can develop without Med26, the organism as a whole dies if the subunit is removed.

“Med26 is essential for multicellular life and is involved in both active and silent genes,” Marr says. “Exactly how is the next big question.”

The Science of Stressing People Out

Ever had that dream where you’re about to take a test or perform in a play or go to a job interview and you are completely, woefully unprepared?

Many of us have that classic nightmare when we’re stressed out. But, for the scientists who study the effect of stress on the body, recreating those unnerving situations in real life is an important part of their research, however sadistic it may sound.

Psychology professor Nicolas Rohleder and his team of graduate and undergraduate researchers use so-called stress tests to study interleukin-6 (IL-6), an inflammatory agent linked to stress and a known contributor to heart disease, diabetes and cancer.

The science of stressing people out has, thankfully, evolved over the years from early tests in which participants were asked to stick their hands in buckets of ice or forced to watch videos of bloody surgeries, to more humane procedures today.

Although stress and its impact on the body have been studied since Austrian-Canadian endocrinologist Hans Selye’s experiments in the 1930s, stress tests weren’t standardized until the 1990s, when Clemens Kirschbaum of the Technische Universität in Germany came up with the Trier Social Stress Test (TSST).

Instead of using ghoulish or physically shocking methods to measure stress response, the TSST is designed to trigger a stress response to a social threat.

Considered the gold standard of stress tests, it has been used around the world in thousands of studies. Rohleder’s lab alone has put more than 1,200 people through the test. Though Rohleder can’t reveal everything in the TSST — it would ruin the stress for participants — he can share the basics.

First, researchers invite participants to sit in a comfy chair in a quiet room to draw blood to establish a baseline. Of course, needles themselves are stressors for some, so researchers attempt to make the experience as relaxing as possible.

Next, participants are introduced to a team of researchers and asked to perform two different kinds of tasks. The first requires interacting with the researchers and the second requires solving a problem. Two researchers observe and time participants performing the tasks.

Credit:  John Coetzee
Credit: John Coetzee

I know problem-solving tasks can be stressful — math certainly stresses me out. But what’s so scary about a social interaction, you might ask. Think about a time you went on a first date, or to a party where you didn’t know many people. Think about your first day of orientation at Brandeis. How did you feel? Were your palms sweaty? Was your heart beating a little faster?

As social creatures, humans need social interactions to survive, says Rohleder. But people who perform poorly in social situations risk being isolated or cast out of the group. Evolutionarily speaking, their genetic longevity may be in danger (though there are exceptions to the rule).

Obviously, researchers can’t expose subjects to life-threatening stressors, but luckily, social stressors provide the next best measure. The risk of social isolation triggers many of the same biological responses as physical threats, and the sympathetic nervous system kicks into gear, cortisol levels spike, and secondary stress systems, such as the inflammatory agent IL-6, activate.

Most of the time, by the end of the TSST, participants’ heart rates are up,  they might be sweating; their adrenalin might be pumping. The test usually stresses researchers out, too, Rohleder says, since they are also in a difficult social situation.

After the test, researchers take another blood sample to measure the biochemical changes in the body caused by stress. Participants can then leave — probably to calm down over a stiff drink.

Future stress test might include social media stressors, like Twitter trolls or nasty Facebook posts but we likely won’t see that in the lab until more people start seeing it in their dreams.

To read about Rohleder’s current research, check out our story on BrandeisNow.

Dogic Lab gets Science cover

On Sept. 5, the Dogic lab’s active matter research was featured on the cover of the journal Science.  Dogic, with a team from Technical University of Munich (TU Munich), Leiden University in the Netherlands, and Syracuse University, created a Frankenstein-like synthetic sac,  stitched together from different kinds of biomolecules, that can move and change shape on its own. It’s called an active nematic vesicle and it could be the first step towards building artificial cells.

So you want to work in a lab? Abby Knecht ’15 offers some advice

Dear freshmen,

You probably learned about RNA transcription in high school but have you ever seen it in action? I spent my summer watching, in real time, as small sections of DNA were converted into RNA for gene expression.

I work in a biochemistry lab at Brandeis that studies transcription under special fluorescent microscopes that allow us to observe single molecules interacting. It’s an incredible feeling to be able to see and study something that is so fundamental for life itself but hidden from our everyday view.

Working in a lab is unlike any science course you’ve taken so far. Science is about puzzles.  It’s about looking at the world, asking questions and finding ways to uncover the answers to those questions. But as one answer is found, more questions invariably pop up and the process continues.

Some of you may find this uncertainty frustrating, and long for the hard facts of lectures. Others may find that you enjoy discovering new things — things no one else has seen and are glad that memorization is not required. Others (like myself) may find that you like science in all its forms and enjoy both.

Whatever group you fall into, you won’t know until you try working in a lab.

Working in a lab is the best way to see how science is really done. You’re on the frontier of discovery. Unlike high school, where lab results are often spoon-fed to you, no one knows what the results will be: that’s why you’re doing the experiment.

Brandeis is a great place for undergraduate research. There are a lot of research labs and undergraduates have a chance to step up and perform their own research. I recommend anyone even remotely interested in science to try working in a lab.

If you want more help applying for labs or deciding whether or not to try it, I advise talking to Hiatt, our on-campus career center, your adviser, or the undergraduate department representative (UDR) in your field of interest.

Good luck!

Abby Knecht is a senior studying Biological Physics at Brandeis University.  She works in the Gelles lab researching the effects of negatively supercoiled DNA on the mechanism of transcription initiation.  When she is not in the lab or studying for one of her many science classes, she is either reading, drawing, or hanging out with friends. 

Making the Case for Cool Science

In 1888, an Austrian botanist dissected a carrot and today, we have LCD TVs. That’s the thing about science, you never know where it will lead.

That botanist was Friedrich Reinitzer, and he observed cholesterol extracted from a carrot that behaved both like a liquid and a solid. It was the first observation of a liquid crystal. Over the next century, it spawned a new field of research and countless technologies.

Would Reinitzer’s research be funded today?

With funding down and competition up, researchers have to fight for every dollar. Even fundamental researchers — the scientists who explore the basic building blocks of life and matter — are often asked to predict how their research could be applied.

At Brandeis, researchers build the foundations for curing disease, developing clean energy and understanding the cosmos. The research goal may be identifying how a particular protein operates in the disease process, or which chemicals won’t act like greenhouse gases in the atmosphere. But we also celebrate research that explores an unknown frontier without knowing where it will lead.

Physicists Zvonimir Dogic and Michael Hagan are doing just that.

Recently, their research into two-dimensional physics was featured in Nature, one of the most respected journals in science. The paper was also authored by Brandeis University graduate students Prerna Sharma and Andrew Ward. Knowing just how their research will be applied is decades down the road. But that doesn’t diminish the cool factor; indeed maybe it heightens it.

In the three-dimensional world, oil and water separate when combined. The smaller droplets of oil merge with larger ones, eventually forming one continuous layer that floats on water.

It’s a basic principle in physics.

But in the two-dimensional world, things work very differently.

Take our cell membranes, a two-dimensional liquid world. Liquid lipid “rafts” float on the surface of membranes, carrying proteins and regulating transport in and out of the cell.

If the lipid rafts behaved like liquid in the three-dimensional world, the individual rafts would coalesce into one larger raft.

But, as the researchers observed, the 2-D world has its own operating system.

To get an inside peek at this microscopic world, Dogic and Hagan needed to scale up, big time. They used long rod-like viruses to create a colloidal membrane — a membrane made up of thousands of smaller particles — like our cell membranes except much, much larger.

To build the lipid rafts, they used shorter rod-like viruses.

When the team put the shorter and longer viruses together, something unexpected happened: instead of separating like oil and water, the short rods self-assembled into smaller droplets of equal size and shape.

And they held that size and shape. Even when the sample started out with different sized droplets, they all equalized.

Even when the team split a droplet, it returned to its original size a few hours later.

Dogic and Hagan discovered droplets don’t coalesce because they actively repel each other, like magnets flipped on one side. A droplet’s chirality — the way its component rods twist in space — creates a distortion in the membrane, which its fellow droplets want to avoid. The effect is long-range, with the droplets avoiding each other as far as 10 diameters away.

The droplets constantly exchange and rearrange their rods to maintain the same size.

In the 3-D world this just doesn’t happen, but our rules don’t apply to the 2-D world.

Dogic and Hagan also discovered that by tweaking the parameters of the droplets — their chirality, for example — they can assemble into different shapes, like long, polymer-like strings or horseshoes.

So what does this all mean?

It means we’re just beginning to lift the curtain on the two-dimensional world. Sure, Dogic and Hagan can speculate about applications like self-healing fabrics or new drug delivery systems, but right now, it’s just cool science. No, it’s fundamental science. And that’s cool.