Rice ’10 discusses new assay for multiple drug resistant TB

[VIDEO] Lisa Rice ’10 presenting at the 6th Annual New England Tuberculosis Symposium held at the Broad Institute on June 28th 2012. Lisa’s undergraduate thesis work in the Wangh lab involved design and construction of a single-tube assay for species identification using the lab’s LATE-PCR and Lights-On/Lights-Off probe technologies.  After graduation she remained in the lab and worked as a very skilled technician on two projects, a single-tube assay for Sepsis, and on the  construction of a single-tube assay for multi-and-extensively drug-resistant tuberculosis (discussed in the video below).  That assay is now beginning validatation in Cape Town, South Africa.

Smiths Detection and Novartis Diagnosics sign deal for LATE PCR technology

Smiths Detection and Novartis Diagnostics have entered an collaboration and license agreement for marketing the Bio-Seeq instrument. This uses LATE PCR technology developed in the Wangh lab at Brandeis and licensed by Smiths Detection.

Dilute-’N’-Go sequencing

Prof. Larry Wangh and his lab are interested in detecting changes in mitochondrial genomic sequences that result from aging, disease, or drugs.  To do this, they use LATE-PCR, an advanced form of asymmetric PCR, to detect mutations in the mitochondria by using multiplexes to study many mitochondrial genes at the same time.  LATE-PCR generates single DNA strands that are easily diluted for sequencing.  In the past. they have only been able to sequence one DNA strand from these multiplex reactions.

In a recent publication in Nucleic Acid Research, staff members Yanwei Jia and John Rice, along with Molecular and Cell Biology grad student Adam Osborne, describe the development of a blocking reagent that allows them to sequence both strands of the product DNA, thus allowing for the easy verification of mutations.

The figure at right shows that without a blocker (BLK), one is not able to obtain the excess (XP) strand sequence from a multiplex reaction.  Using a blocker one is able to get not only the limiting (LP) strand, but also the excess strand from the same multiplex

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